Dr vg malathi, iari tomato leaf curl palampur virus. Chilli leaf curl palampur virus is a distinct begomovirus. Jun 01, 2016 these symptoms are different from those that observed for other begomoviruses previously reported to infect cucurbit crops in iran. We were able to identify a third begomovirustomato. In india, chilli has been reported to be infected by several begomoviruses, namely chilli leaf curl virus chilcv, tomato leaf curl new delhi virus tolcndv and tomato leaf curl joydebpur virus khan et al. Molecular characterization of tomato leaf curl palampur. Infectious clones of tomato leaf curl palampur virus with a defective dna b and their pseudorecombination with tomato leaf curl new delhi virus. Av2 protein of tomato leaf curl palampur virus promotes. Hence this datasheet will refer only to the tylcv complex, not to the other, different, tomato. Sep 26, 2017 genotype tomato leaf curl new delhi virus dsi tomato leaf curl bangalore virus dsi tomato leaf curl palampur virus dsi 30 dpi 60 dpi 30 dpi 60 dpi 30 dpi 60 dpi kashi vishesh 3. The present investigation entitled was carried out to identify the sources for chilli leaf curl virus chilcv resistance. Association of tomato leaf curl new delhi virus, betasatellite, and. Chilli leaf curl virus management chilcv disease is the most destructive virus disease in terms of incidence and yield loss.
Tomato leaf curl disease tlcd and and tomato yellow leaf curl tylcd is caused by a number of begomovirus species that collectively threaten tomato production. Detection and characterization of tomato leaf curl new delhi virus association with mosaic disease of ivy gourd coccinia grandis l. This document is fact sheet pp184, one of a series of the plant pathology department, florida cooperative extension service, institute of food and agricultural sciences, university of florida. We were able to identify a third begomovirustomato leaf curl palampur. International journal of biotechnology and bioengineering. Yellow leaf curl virus, for example is transmitted by whiteflies, and new leaves on infected plants become cupped and pale green in color.
Complete sequences of tomato leaf curl palampur virus isolates. Association of tomato leaf curl palampur virus with yellow mosaic. Association of virus complex with capsicum under protected cultivation in himachal pradesh and implications in its management himachal journal of agricultural research 421. Tomato yellow leaf curl virus tylcv is a dna virus from the genus begomovirus and the family geminiviridae. Asymmetric synergism and heteroencapsidation between two bipartite begomoviruses, tomato leaf curl new delhi virus tolcndv and tomato leaf curl palampur virus tolcpalv virus res 174.
We have previously shown the association of zucchini yellow mosaic virus zymv, a potyvirus, with severe leaf curl disease of muskmelon in pakistan. Caused by whitefly transmitted geminiviruses wtg how many viruses are there in. Jan 19, 2018 tomato leaf curl palampur virus tolcpalv is a bipartite begomovirus that infects tomato, cucurbits and beans in asia,14,15. Tomato leaf curl new delhi virus tolcndv is a bipartite, whiteflytransmitted, begomovirus which was first described on. We were able to identify a third begomovirus tomato. Tomato yellow leaf curl virus management for homeowners. Multiple begomoviruses found associated with cotton leaf curl disease in pakistan in early 1990 are back in cultivated cotton.
Viruses free fulltext chickpea chlorotic dwarf virus. Tomato yellow leaf curl virus resistance by ty1 involves increased cytosine methylation of viral genomes and is compromised by cucumber mosaic virus infection. Molecular characterization of tomato yellow leaf curl. Frontiers implication of the whitefly bemisia tabaci. Several leaf curl virus has been reported for infecting tomato as tomato leaf curl sudan virus jarullah et al. Capsicum frutescens chilli plants exhibiting leaf curl symptoms, collected from palampur, himachal pradesh region of india, were found associated with a. Recently there has been a lot of publicity concerning the latest imported pest problem of. Curly top disease of tomato oklahoma state university. Tomato leaf curl palampur virus tolcpalv is a whiteflytransmitted, bipartite begomovirus. Tomato yellow leaf curl virus tylcv is a circular single strand dna begomovirus, which causes one of the most important diseases in tomato worldwide. Infectious clones of tomato leaf curl palampur virus.
Namrata jaiswal postdoctoral researcher purdue university. Molecular evidence for association of tomato leaf curl new delhi virus with leaf curl disease of papaya carica papaya l. Ac4 protein of tomato leaf curl palampur virus is an rna. General information about tomato leaf curl new delhi virus tolcnd eppo global database.
Voigt in north india venkataravanappa venkataravanappa1, lakshminarayana reddy c. The westward journey of alfalfa leaf curl virus read by qxmd. Nucleotide sequence analysis of dnaa showed that it shared highest 97. Tomato yellow leaf curl tomato yellow leaf curl virus tylcv in tomato, symptoms are more severe when infected at an earier age and vary somewhat among the different cultivars. Detection and characterization of tomato leaf curl new delhi virus. In india, chilli has been reported to be infected by several begomoviruses, namely chilli leaf curl virus chilcv, tomato leaf curl new delhi virus tolcndv and tomato leaf. Zaidi plant virology laboratory, council of scienti. Pdf complete sequences of tomato leaf curl palampur virus. Backgroundtomato leaf curl palampur virus tolcpmv is a bipartite begomovirus which has been reported from india and iran but infectious clones have not been obtained. Evolutionary and molecular aspects of indian tomato leaf. We were able to identify a third begomovirustomato leaf curl palampur virus tolcpmv, from pumpkin showing typical symptoms of the disease at varanasi based on the sequence of complete dnaa genome of the virus. Association of tomato leaf curl palampur virus with yellow mosaic disease of armenian cucumber cucumis. The tomato leaf curl new delhi virus tolcndv genus begomovirus, family geminiviridae represents an important constraint to tomato production, as it causes the most predominant and economically important disease affecting tomato in the indian subcontinent.
Tomato leaf curl palampur virus tolcpmv is a bipartite begomovirus which has been reported from india and iran but infectious clones have. Infectious clones of tomato leaf curl palampur virus with. Molecular characterization and infectivity of a tomato leaf. Viruses free fulltext tomato leaf curl new delhi virus. These results indicate that the virus infecting pumpkins cucurbita moschata is considered as a strain of tomato leaf curl palampur virus infecting tomato plants in india jaiswal et al. Tomato leaf curl palampur virus tolcpmv is a bipartite begomovirus which has been reported from india and iran but infectious clones have not been obtained. Curling tomato leaves may be a sign of a viral infection. Rana department of plant pathology, college of agriculture, csk hpkv, palampur 176062 hp.
Infectious clones of tomato leaf curl palampur virus with a. Jahangir heydarnejad, azadeh mozaffari, hossain massumi, roya fazeli, alistair j a gray, sandra meredith, francisco lakay, dionne n shepherd, darren p martin, arvind varsani archives of virology 2009, 154 6. Tomato leaf curl palampur virus tolcpv, a begomovirus species, has been reported to cause mosaic and leaf curl disease in tomato and cucurbit crops kumar et al. Screening of genotypes against the chilcv is crucial to select the appropriate genotypes to get the successful crop production under the disease pressure.
Tomato leaf curl palampur virus tolcpalv is a whiteflytransmitted. Tomato leaf curl new delhi virus and squash leaf curl china virus are known to be associated with this disease in india. Later in 2009, tolcpmv was reported to cause an epidemic disease in cucurbit and. Discovery of a novel circular dna virus in the forbes sea star, asterias forbesi. A sequencing analysis of the full genome revealed that it is a strain of tomato leaf curl palampur virus genbank id. Tomato leaf curl palampur virus tolcpalv is a bipartite begomovirus in geminiviridae family. Previous studies have shown that the transmission, retention, and circulation of tylcv in its vector involves. Leaf curl, yellow leaf curl hosts tomato, pepper, eggplant, okra, tobacco, beans, cotton, cucurbits, citrus, and other crops as secondary. It is responsible for significant reduction in the crop yield and quality. Tomato leaf curl palampur virus30, bhendi yellow vein mosaic virus 28. Molecular characterization and infectivity of a tomato.
Evolutionary and molecular aspects of indian tomato leaf curl. Department of plant protection, college of agriculture, shahid. A name tomato leaf curl palampur virus tolcpmv was proposed for this new species kumar et al. Tomato leaf curl palampur virus tolcpalv is a bipartite begomovirus that infects tomato, cucurbits and beans in asia,14,15. Pumpkin cucurbita moschata samples showing yellow vein mosaic disease in varanasi region were identified with begomovirus infection using pcr amplification. Tomato leaf curl palampur virus tolcpalv dr vg malathi, iari. Tomato is not a preferred host for the beet leafhopper. Ac4 protein of tomato leaf curl palampur virus is an rna silencing suppressor and a pathogenicity determinant. Leaf curl diseases caused by tomato leaf curl palampur virus tolcpalv is responsible for considerable yield losses and pathogenicity of virus is attributed to the expression of a viral protein, av2. The tomato leaf curl new delhi virus tolcndv genus begomovirus, family geminiviridae represents an important constraint to tomato production, as it causes the most predominant. The complete dnaa and dnab genomic components of the causative virus were cloned and sequenced. Jan 19, 2018 tomato leaf curl palampur virus tolcpalv is a bipartite begomovirus that infects tomato, cucurbits and beans in asia 15. Infectious clones of tomato leaf curl palampur virus with a defective dna b and their pseudo. We were able to identify a third begomovirus tomato leaf curl palampur virus tolcpmv, from pumpkin showing typical symptoms of the disease at varanasi based on the sequence of complete dnaa genome of the virus.
Tomato yellow leaf curl virus tylcv is a tomato solanum lycopersicuminfecting plant virus transmitted by whitefly bemisia tabaci 1,2. Accordingly, tomato yellow leaf curl virus tylcv is the name of the virus isolated in israel. Av1 and av2 are present on the virion sense strand, and ac1, ac2, ac3 and ac4 are present on the virion antisense strand. Though it can take up to three weeks before any symptoms develop, the most common indicator of the disease is the yellowing and upward curling of the leaves. We report here that an ongoing tlcd and tylcd epidemic in iran is caused by variants of tomato leaf curl palampur virus tolcpmv, a newly proposed begomovirus species previously only detected in india. Analysis of watermelon chlorotic stunt virus and tomato leaf curl palampur virus mixed and pseudorecombination infections. Mar 16, 2011 tomato leaf curl new delhi virus and squash leaf curl china virus are known to be associated with this disease in india. Association of virus complex with capsicum under protected.
Characterization of tomato leaf curl new delhi virus and. Molecular characterization of tomato leaf curl palampur virus. Tomato yellow leaf curl virus broad mites one easy way to prevent this problem is by planting tomato species that are meant to grow in your climatic conditions and also by selecting diseaseresistant varieties. Jyothsna p pathologist bayer crop science linkedin. Here, we demonstrated that ectopic expression of av2 from a potato virus x pvxbased vector. Full text of infectious clones of tomato leaf curl palampur. Characterization of tomato leaf curl new delhi virus and dna. Infectious clones of tomato leaf curl palampur virus with a defective. Frequent occurrence of tomato leaf curl new delhi virus in cotton.
Chilli leaf curl palampur virus is a distinct begomovirus species associated with a betasatellite y. However, in other begomoviruses, the ac4 is a suppressor and a. I coat protein a vi gene, gq225738 and tomato leaf. Normally this virus is transmitted through whiteflies or through infected transplants. However, in recent years, tolcndv has been fast extending its host range and spreading to new geographical regions, including the. However, the severity of symptoms in the field and yield losses led us to. Tomato leaf curl disease tolcd is manifested by yellowing of leaf lamina with upward leaf curl, leaf distortion, shrinking of the leaf surface, and stunted plant growth caused by tomato leaf curl virus tolcv. The present study describes the association of a distinct begomovirus species and a. The study revealed that the amount of total protein declined in the infected leaves but elevated up to 5 % in the fruits of infected plants.
Multiple begomoviruses found associated with cotton leaf curl. Watermelon chlorotic stunt virus and tomato leaf curl palampur virus. Fahsbender e, hewson i, rosario k, tuttle ad, varsani a, breitbart m. Av2 protein of tomato leaf curl palampur virus interacts. Tomato leaf curl palampur virus tomato leaf curl joydebpur virus. Management of tomato yellow leaf curl virus tylcv in tomato in north florida1 tim momol, steve olson, joe funderburk, and richard sprenkel2 1. The genus begomovirus under family geminivirideae, consists of a group of viruses exclusively transmitted by whitefly b. Tomato leaf curl new delhi virus on zucchini cucurbita pepo courtesy. Indian cassava mosaic virus icmv, plum pox virus ppv and tomato leaf curl palampur virus tlcpv infection modified the composition of nutritive and bioactive compounds of plant host, momordica charantia, prunus domestica and cucurbita moschata, respectively 15, 18, 19.
Tomato yellow leaf curl virus tylcv is a singlestranded ssdna begomoviruses that causes severe damage to tomato and several other crops worldwide. Tylcv causes the most destructive disease of tomato, and it can be found in. Tomato yellow leaf curl virus tylcv university of florida. Phytochemicals and antioxidative enzymes defence mechanism on. Cotton leaf curl disease clcud is the major biotic constraint to cotton. The formatted products tool fpt is a process that captures all line items in a formatted template, sends those line items through a standardization process, and allows the user to send their product and catalog data directly to. Here, we demonstrated that ectopic expression of av2 from a potato virus x. Tomato leaf curl disease tlcd and and tomato yellow leaf curl tylcd is caused by a number of begomovirus species that collectively threaten tomato production worldwide. Tylcv is exclusively transmitted by the sweetpotato whitefly, bemisia tabaci in a persistent circulative and propagative manner.
Complete sequences of tomato leaf curl palampur virus. Temporal and spatial dynamics of tomato leaf curl disease at. Its genome has two orfs in the virion sense v1 and v2 and four orfs in the antisense c1, c2, c3 and c4 orientation, separated by an intergenic region ir. Tomato leaf curl disease tolcd is manifested by yellowing of leaf lamina with upward leaf curl, leaf distortion, shrinking of the leaf surface, and stunted plant growth caused by tomato leaf. Av2 protein of tomato leaf curl palampur virus promotes systemic. Tomato yellow leaf curl virus tylcv is one of the most studied plant viral pathogens because it is the most damaging virus for global tomato product. Full text of infectious clones of tomato leaf curl. Phytochemical composition and antioxidative enzyme levels were compared in infected and healthy plants. Management of tomato yellow leaf curl virus tylcv in. Based on this data, the new chilliinfecting begomovirus in this study is considered to be a member of a new species for which the name chilli leaf curl palampur virus chilcpav is proposed. Tomato leaf curl new delhi virus on zucchini cucurbita pepo.
Tomato yellow leaf curl virusis causes a novel disease of com mon bean and severe epidemics in tomato in spain. Indian virus is a provisional strain of cuculbita pepo begomovirus eu366164 or tomato leaf curl palampur virus gq225738. Detection and characterization of tomato leaf curl new delhi. The present study was designed to elucidate role of av2 in virus infection and characterize its interaction with tomato fbox kelch protein kfb. Complete sequences of tomato leaf curl palampur virus isolates infecting cucurbits in iran. In the present study, using computational methods we explored the evolutionary and molecular prospects of viral coat protein derived from an isolate of vadodara district, gujarat. Further, tr ansreplication involving tolcndv and isolates of a tomatoinfecting monopartite begomovirus t omato leaf curl ranchi virus and its associated. Screening of popular indian chili pepper capsicum annuum l. Tomato leaf curl kerala virus tolckev dr sk mukherjee, icgeb. Av2 protein of tomato leaf curl palampur virus interacts with. Leaf curl diseases caused by tomato leaf curl palampur virus tolcpalv is responsible for considerable yield losses and pathogenicity of virus is attributed to the expression of a viral.
994 1512 129 335 861 538 155 418 488 624 1174 30 117 134 1075 1445 144 714 638 1488 1227 156 1336 372 666 1310 403 910 530 1211 1103 1313 1209 1370 996 415 790 1310 1090 1023 1145 492 1462 41 1116